leptin receptor protein level (Santa Cruz Biotechnology)
Structured Review

Leptin Receptor Protein Level, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 347 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/leptin+receptor+protein+level/Leptin/10__1074_slash_jbc__m401546200-78-0-15
Average 95 stars, based on 347 article reviews
Images
1) Product Images from "Impaired Activation of Phosphatidylinositol 3-Kinase by Leptin Is a Novel Mechanism of Hepatic Leptin Resistance in Diet-induced Obesity"
Article Title: Impaired Activation of Phosphatidylinositol 3-Kinase by Leptin Is a Novel Mechanism of Hepatic Leptin Resistance in Diet-induced Obesity
Journal: Journal of Biological Chemistry
doi: 10.1074/jbc.m401546200
Figure Legend Snippet: FIG. 7. Effects of diet-induced obesity on hepatic leptin recep- tor expression. Livers were isolated from 18 h of fasted lean or diet-induced obese rats. Leptin receptor protein and leptin receptor mRNA were measured by Western blot and RPA, respectively, as de- scribed under “Experimental Procedures.” The figure shows represent- ative autoradiographs of ObRa protein and ObRtot mRNA in liver from lean (LEAN) and diet-induced obese (OBESE) rats. n a minimum of 6 livers/group.
Techniques Used: Expressing, Isolation, Western Blot
Related Articles
Western Blot:Article Title: Impaired Activation of Phosphatidylinositol 3-Kinase by Leptin Is a Novel Mechanism of Hepatic Leptin Resistance in Diet-induced Obesity Article Snippet: For quantification of phosphorylation of IRS-1, 500 g of liver protein was immunoprecipitated overnight with -IRS-1 and the immunocomplexes were separated by 7.5% SDS-PAGE and probed with -phosphotyrosine (PY20, 1:1000, CRP Inc., Denver, PA) or -phosphoserine-IRS-1 (Ser-612, 1:1000, Cell Signaling Beverly, MA) antibodies. .. |